HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hiatt, C. W. Articles by Baron, S. Search for Related Content PubMed PubMed Citation Articles by Hiatt, C. W. Articles by Baron, S.

Inactivation of Viruses by the Photodynamic Action of Toluidine Blue

From the Department of Health, Education and Welfare, Public Health Service, National Institutes of Health, Division of Biologics Standards, Bethesda, Maryland

Abstract

In a study of the rates of inactivation of 11 different viruses by the photodynamic action of toluidine blue and visible light, a wide range of variation was encountered. Relative velocity constants, determined under standard conditions of dye concentration and light intensity, ranged from 1.0 for T3 coliphage and vaccinia virus to less than 0.001 for poliovirus and Coxsackie B2 and appeared to correspond at least approximately with accepted taxonomic groupings.

According to a hypothetical mechanism for this reaction, the dye combines at sites essential for infectivity, providing "marked targets" for the destructive action of the light energy. The dye then acts as a photosensitizer by absorbing light of specific quantum energy and transferring the energy increment to the virus particle at the site of combination.

This article has been cited by other articles:

				F. Rapp and J.-L. H. Li Demonstration of the Oncogenic Potential of Herpes Simplex Viruses and Human Cytomegalovirus Cold Spring Harb Symp Quant Biol, January 1, 1974; 39(0): 747 - 763. [Abstract] [PDF]