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A Modified Technique of Immunoelectrophoresis Facilitating the Identification of Specific Precipitin Arcs^1,2,

From the Department of Medicine, Francis Delafield Hospital, and the College of Physicians and Surgeons, Columbia University, New York, New York

Abstract

A modification of the technique of immunoelectrophoresis has been developed which facilitates the identification of individual precipitin arcs in complex mixtures of proteins such as human serum. This modified procedure consists of the concurrent reaction of the antiserum with the electrophoretically separated primary antigen solution (e.g., whole serum) and with a known protein constituent. Examples are presented of the application of the technique to the identification of the components in Cohn Fraction IV-7 (primarily the iron-binding globulin), ceruloplasmin, and the urinary proteins in a case of myeloma.

Footnotes

This work has been supported by a grant from the National Cancer Institute, National Institutes of Health, U. S. Public Health Service (CY-2332).

² A preliminary report of this work was presented at the 43rd Annual Meeting of the American Association of Immunologists, April 1959, Atlantic City, N. J. (1).

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