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The Journal of Immunology, 1960, 84: 6-10.
Copyright © 1960 by The American Association of Immunologists, Inc.
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Purification of Rabies Antibodies in Horse Serum and Diagnostic Importance of the Fluorescent Antibody Technique

M. Etchebarne, P. G. Bernal and G. R. Leyton

From the Physical-Chemistry Section of the Bacteriological Institute, Santiago, Chile

Abstract

1. Purified horse rabies antibody has been prepared by the isoelectric fractional alcohol precipitation method of Nichol and Deutsch, with a yield of 54%. Neutralizing antibody in the final preparation is found mainly in the {gamma}-2-G fraction.
2. Modifications of the method of Coons were used for conjugating the purified antirabies horse antibody with fluorescein isocyanate. Staining of brain smears from rabies-infected dogs and mice with fluorescent antibody showed the diagnostic usefulness of this method in a type of communicable disease in which prompt information is most urgent.






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