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Studies on the Immobilization Antigens of Paramecium

II. Isolation

From the Biology Division, University of Pennsylvania, Philadelphia, Pennsylvania

Abstract

Good yields of immobilization antigen have been obtained by extracting paramecia in a dilute salt solution containing 15% alcohol for 1 hr at 2°C. The extract is purified by low pH treatment followed by fractional precipitation with ammonium sulfate. Data on solubility, electrophoresis, ultracentrifugation, along with serologic criteria indicate that the material is relatively pure immobilization antigen.

Footnotes

¹ Aided by grants to the author from Phi Beta Psi Sorority and Public Health Service, National Institutes of Health, and by grants to Professor T. M. Sonneborn from the Rockefeller Foundation, Atomic Energy Commission, and Narional Science Foundation. The major portion of the work was done while on sabbatical leave at Indiana University, Bloomington, Indiana.