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Allergic Encephalomyelitis

The Extraction of Highly Active Fractions from Bovine Spinal Cord¹

From the Kentucky Child Health Foundation Laboratory, Department of Pediatrics, University of Louisville School of Medicine, Louisville

Abstract

1. By extraction of whole moist bovine spinal cord with petroleum ether, a fraction can be obtained which is highly encephalitogenic when combined with Freund adjuvants and injected intracutaneously into guinea pigs.

2. Fractionation with acetone, chloroform and methanol yields a preparation which causes a high rate of allergic encephalomyelitis when injected in 5- amounts. This material contained 6.3% nitrogen.

3. Further fractionation yields a fraction (almost completely protein) which is found to be highly encephalitogenic in 2- amounts.

Footnotes

¹ Aided by grants from National Institute of Neurological Diseases and Blindness and the National Multiple Sclerosis Society.