HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Nisonoff, A. Articles by Pressman, D. Search for Related Content PubMed PubMed Citation Articles by Nisonoff, A. Articles by Pressman, D.

The Similar Specificity of the Combining Sites of an Individual Antibody Molecule

From the Department of Biochemistry Research, Roswell Park Memorial Institute, Buffalo, New York

Abstract

Antiserum prepared in rabbits against bovine--globulin (BGG) to which p-azobenzoate groups were coupled contained precipitins directed against BGG and against the haptenic group. Treatment of a -globulin fraction of the antiserum with an excess of a solid adsorbent capable of removing antibody with anti-BGG combining sites did not decrease the number of antihapten sites. The failure to remove antihapten activity was shown by direct binding measurements with labelled p-iodobenzoate.

It is concluded that essentially none of the antihapten combining sites are on molecules having an anti-BGG site; i.e., that heteroligating antibody is absent. The results are discussed from the standpoint of the mechanism of antibody formation.