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Studies on Ragweed Pollen

IV. Isolation and Characterization of an Allergenic Fraction from Ragweed Pollen^1,2,

From the Department of Chemistry, McGill University, and the Division of Immunochemistry and Allergy, McGill University Clinic, Royal Victoria Hospital, Montreal, Canada

Abstract

1. An allergenically active fraction was isolated from ragweed pollen which was 50 times as active, on a weight basis, as the dialyzed residue of the whole water soluble extract of ragweed pollen.

2. This material consisted of a straw colored pigment and peptide(s) composed of eight amino acids: lysine, arginine, glycine, glutamic acid, hydroxyproline, valine, alanine and norleucine. It did not contain any carbohydrate.

3. The allergenic activity was destroyed after hydrolysis with 6 N HCl at 100°C for 15 min, but was not diminished on standing in 6 N HCl at 25°C for 8 hr or if heated in boiling water at pH 7.0 for 14 hr.

4. The active material gave positive ring tests with both rabbit and goat antiragweed pollen sera.

5. The available evidence suggests that peptide(s) may account for all the allergens in ragweed pollen.

Footnotes

¹ Submitted in partial fulfillment of the requirements for the degree of Ph.D. in the Department of Biochemistry, McGill University (M. R.).

² This study was supported by grants from the Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md., and the Department of Health and Welfare, Canada. We are also indebted to the Charles E. Frosst Co., Montreal, for financial assistance.

³ Present address: Department of Chemistry, University of Indiana, Bloomington, Indiana.