HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Glenn, W. G. Search for Related Content PubMed PubMed Citation Articles by Glenn, W. G.

Some Considerations in Agar Column Diffusion Analyses¹

From the Department of Microbiology-Cellular Biology, School of Aviation Medicine, USAF, Randolph Air Force Base, Texas

Abstract

1. By direct photometry the zone density of human serum albumin homologous systems diffusing in agar columns varied inversely with the initial antigen concentrations up to 150 hr reaction time. Such observation is a corollary to the general rule that the antibody concentration alone governs the zone density.

2. Nonspecific substances added to human serum albumin increased the zone densities; whereas the removal of albumin from rabbit antiserum decreased zone densities. This effect is probably due to altered solubilization of the precipitates.

3. Micro-Kjeldahl analyses of whole diffusion patterns containing several zones will provide an absolute measure for indicating the reactivity of a complex precipitin system. Such measure is not presently obtainable by the classic quantitative precipitin method.

Footnotes

¹ A part of this report was presented by invitation at the session on Diffusion Methods in Immunological Research, VII International Congress for Microbiology, Stockholm, Sweden, August 4, 1958.