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Immunochemical Studies of Poliovirus

IV. Alteration of the Immunologic Specificity of Purified Poliomyelitis Virus by Heat and Ultraviolet Light^1,2,

From the Department of Microbiology, The Johns Hopkins University, School of Medicine and School of Hygiene and Public Health, Baltimore, Maryland

Abstract

Exposure of purified poliovirus, Fraction SDG^D, which comprises ID⁺ and NID⁺ particles, to heat at 56°C or to ultraviolet light, results in loss of infectivity and in a change in immunologic specificity, manifested by a loss of reactivity in complement fixation tests with suitable convalescent phase sera from poliomyelitis patients, and a concomitant appearance of reactivity with certain acute patient phase sera.

The rate of loss of infectivity on heating at 56°C was about the same as the rate of conversion of immunologic specificity. On the other hand, upon exposure to ultraviolet light the rate of loss of infectivity was far greater than the rate of serologic change.

The altered antigen has been designated as denatured D-antigen. We consider it likely that the NIC^- particles described in the previous report (9) are immunologically identical with, or similar to, denatured D-antigen.

Footnotes

¹ Aided by a grant from the National Foundation for Infantile Paralysis, Inc.

² Preliminary report of part of this work was presented at the Federation Meetings, April 15, 1957.