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Immunization with an Induced Avirulent Auxotrophic Mutant of Pseudomonas Pseudomallei¹

From the Naval Biological Laboratory, School of Public Health, University of California, Berkeley, California

Abstract

A virulent strain of Pseudomonas pseudomallei was subjected to ultraviolet irradiation and a mutant clone requiring preformed adenine or hypoxanthine for growth was isolated. The imposition of auxotrophism was associated with a striking decline in virulence for mice which could be restored by permitting the strain to revert to purine independence in vitro. The mutant persisted in mice without detected reversions or or untoward effects for approximately 20 days following inoculation of 10⁷ cells. After repeated inoculations, a significant immune response was demonstrated by parenteral challenge with diverse strains of this species. However, animals so immunized remained susceptible to lethal respiratory infection.

Footnotes

¹ This work was sponsored by the Office of Naval Research, U. S. Navy, under a contract between the Office of Naval Research and the Regents of the University of California.

² Opinions expressed in this report are not to be construed as reflecting the views of the Navy Department or of the naval service at large (Article 1252, U. S. Navy Regulations, 1948). Reproduction in whole or in part is permitted for any purpose of the United States Government.

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