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Immunochemical Studies of Poliovirus

III. Further Studies on the Immunologic and Physical Properties of Poliovirus Particles Produced in Tissue Culture^1,2,

From the Department of Microbiology, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland

Abstract

Through fractionation in the ultracentrifuge of partially purified concentrated poliovirus from monkey kidney tissue cultures, and by infectivity and serologic assay of the fractions so obtained, evidence has been obtained for the existence of four distinct viral particles. These have been designated ID⁺, NID⁺, NID^- and NIC^-, representing, respectively, infectious virus particles possessing immunologic D specificity, and a sedimentation coefficient of about 160 S, noninfectious particles of D specificity and 160 S, noninfectious particles of D specificity exhibiting a sedimentation coefficient of 80–90 S, and noninfectious particles of C specificity exhibiting a sedimentation coefficient of 80–90 S. The designation of C and D specificity refers to reactivity in complement fixation tests with suitable acute or convalescent phase sera, respectively.

Footnotes

¹ Aided by a grant from the National Foundation for Infantile Paralysis.

² A preliminary report of part of this work was presented at the New York Academy of Sciences Meeting, January 7, 1957 and at the Federation Meetings, April 15, 1957.