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The Journal of Immunology, 1958, 81: 292-296.
Copyright © 1958 by The American Association of Immunologists, Inc.
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Studies on Serum Bactericidal Activity

Interrelationships of Heparin, Citrate, Protamine and X-Irradiation on Serum and Plasma Bactericidal Activity Against Bacillus Subtilis1

David M. Donaldson and Stanley Marcus

From the Department of Bacteriology, Brigham Young University and the University of Utah College of Medicine, Provo and Salt Lake City, Utah

Abstract

The intravenous administration of heparin into rabbits caused a temporary depression in the normal serum bactericidal activity. Serum and plasma exhibited less bactericidal activity than normal if either heparin or citrate was added to the blood in vitro immediately after withdrawal and prior to coagulation. In contrast, neither heparin nor citrate had a demonstrable effect on the serum bactericidal activity when added to the separated serum in vitro. The concentrations of citrate that inhibited bactericidal activity were anticomplementary when the complement titrations were carried out in saline. The heparin concentration used did not show a measurable anticomplementary effect with the test system employed.

The depression of bactericidal activity caused by the in vitro or in vivo addition of heparin to blood was partially reversed by the addition of protamine sulfate. The x-irradiation induced depression of bactericidal activity was not reversed by single or multiple injections of protamine sulfate.

Footnotes

This study was supported by research grant E-413 from the National Institute of Allergy and Infectious Diseases, Public Health Service.






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