HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Tsuji, F. I. Articles by Davis, D. L. Search for Related Content PubMed PubMed Citation Articles by Tsuji, F. I. Articles by Davis, D. L.

Effect of Antibodies on the Luminescence of Achromobacter Fischeri as a Criterion of Cellular Penetration^1,2,

From the Research Laboratory, Veterans Administration Hospital, and Department of Biophysics, University of Pittsburgh, ³ Pittsburgh, Pennsylvania

Abstract

Rabbit antisera prepared against whole cell, cell-free extract, and partially purified luciferase of Achromobacter fischeri contain antibodies that are able to react with and inactivate a light emitting component, presumably bacterial luciferase, in cell-free extracts of the organism. Since the same antisera are without light inhibiting effect on live cells, it is postulated that antiluciferase antibodies are not able to penetrate the cell to inactivate luciferase.

Footnotes

¹ Supported in part by a grant (E-1858) from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.

² Presented in part before the Annual Meeting of the Federation of American Societies for Experimental Biology, Philadelphia, Pennsylvania, April, 1958.

³ Publication No. 54 of the Department of Biophysics.