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Differences in the Antigenic Components of Sera of Individual Rabbits as Shown by Induced Isoprecipitins¹

From the Department of Health, Education and Welfare, Public Health Service, National Institutes of Health, National Institute of Allergy and Infectious Diseases, Laboratory of Immunology, Bethesda, Maryland

Abstract

Differences in the antigenic components of sera of individual rabbits have been studied by the induction of isoantibodies. For this purpose, rabbits were injected with normal rabbit sera plus paraffin-oil type adjuvants. The titers of isoantibodies can be estimated by precipitin reactions in agar gel and by passive cutaneous anaphylaxis in the guinea pig. Isoprecipitins were found to serum antigens with electrophoretic mobilities corresponding to -, -, and -globulins.

The sera of 90 normal rabbits were tested by diffusion in gel tubes with 6 rabbit antisera to rabbit sera; some tubes had no precipitin bands and others had one or two precipitin bands. When the reactions between each normal rabbit serum and the 6 rabbit antisera were compared, the 90 rabbits could be grouped into 13 groups on the basis of the presence or absence of precipitin bands and into 30 groups by taking the number of bands into consideration.

The isoantibodies did not react with red blood cell antigens as determined by agglutination tests and adsorption experiments.

Footnotes

¹ Presented in part at the annual meeting of the Federation of American Societies for Experimental Biology, Philadelphia, Pennsylvania, April 14–18, 1958.

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