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Purification of the Toxin of Pasteurella Pestis by Continuous-Flow Paper Electrophoresis^1,2,

From the George Williams Hooper Foundation, University of California, San Francisco, California and The Viral and Rickettsial Disease Laboratory, California State Department of Public Health, Berkeley, California

Abstract

Continuous flow electrochromatography was an effective method for seperating the toxin of Pasteurella pestis from crude extracts. Large amounts of material were processed and a fine degree of fractionation achieved with no evidence of toxin loss. The number of antigens was reduced from 8 to 10 in the crude extract to 3 in the final product. The intravenous LD₅₀ of the purified toxin in the mouse was 0.1 to 0.3 µg as compared to an LD₅₀ of 5 to 15 µg for the crude extract. Toxin from the attenuated strain E.V. 76 behaved similarly in every way tested to the toxin isolated from the highly virulent strain 195/P.

The immunogenic properties of purified toxin from virulent and attenuated strains of P. pestis are under investigation.

Footnotes

¹ This work was done under the sponsorship of the Commission on Immunization of the Armed Forces Epidemiological Board and was supported in part by the Office of the Surgeon General, Department of the Army.

² This paper was submitted jointly with the accompanying paper by Samuel J. Ajl, James Rust, Jr., Donald Hunter, Jeanette Woebke and Donald F. Bent, Since the 2 studies were carried out concurrently and with active mutual contact.