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Inactivation of Complement by Egg Albumin-Rabbit Antiegg Albumin

II. INHIBITION BY SERUM CONSTITUENTS¹

From the Institute of Microbiology, Rutgers, The State University, New Brunswick, New Jersey

Abstract

Possible reasons for differences in the degree of inactivation of guinea pig, human, and pig C' by a fixed quantity of an antigen-antibody system were investigated. It was found that a relatively high concentration of inhibitors in pig serum could account for the absence of fixation of pig C' by small quantities of antigen-antibody which sufficed to inactivate guinea pig C'. These inhibitors in pig serum are fairly stable at pH 4.2. Hemolysins in pig serum are not involved in the inhibition of C' fixation. Human serum was also found to contain less active inhibitors than those in pig serum.

An increase of the relative concentration of C'₄ in pig serum did not increase the degree of fixation of pig C'. However, fixation of guinea pig C' in the presence of heated pig serum resulted in a marked decrease in the inactivation of both C' and C'₄.

Footnotes

This work was supported in part by grants from the National Science Foundation and the Armed Forces Epidemiological Board, Office of the Surgeon General, Department of the Army.

² Waksman-Farmitalia Postdoctoral Fellow from the Istituto di Patologia Generale, Universitá di Napoli.