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The Journal of Immunology, 1958, 80: 349-355.
Copyright © 1958 by The American Association of Immunologists, Inc.
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Studies of the Inactivation of Phage by the Properdin System

II. Quantitative assay of phage-neutralizing activity

James L. Barlow1, Helen Van Vunakis and Lawrence Levine2

From the Division of Laboratories and Research, New York State Department of Health, Albany, and the Department of Microbiology, Albany Medical College

Abstract

Details are given of a method for measuring the neutralizing activity of human serum using bacteriophage as an indicator. The technique measures activity in terms of the 50% neutralizing unit and yields precise and reproducible results. Evidence is presented which suggests that the phage-neutralizing activity might be a reflection of properdin levels.

Footnotes

1 Taken in part from a dissertation to be submitted to the Department of Microbiology, Albany Medical College, Union University, in partial fulfillment of the requirements for the Doctor of Philosophy degree.

2 Present address: Department of Biochemistry, Brandeis University, Waltham, Massachusetts.




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