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Studies of the Inactivation of Phage by the Properdin System

I. EVIDENCE FOR COMPLEMENT, PROPERDIN AND MAGNESIUM REQUIREMENTS

From the Division of Laboratories and Research, New York State Department of Health, Albany, and the Department of Microbiology, Albany Medical College

Abstract

Properdin, complement and Mg⁺⁺, the known constituents of the properdin system, are necessary for neutralization of T_2r⁺ coliphage by human serum. Certain polysaccharides and lipopolysaccharides annul the neutralization. Phage neutralization is pH, ionic strength, and temperature dependent.

Footnotes

¹ Taken in part from a dissertation to be submitted to the Department of Microbiology, Albany Medical College, Union University, in partial fulfillment of the requirements for the Doctor of Philosophy degree.

² Present address: Department of Biochemistry, Brandeis University, Waltham, Massachusetts.

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				K. M. COWAN Interpretation of Properdin Levels Determined by Phage Neutralization Technique Science, October 3, 1958; 128(3327): 778 - 779. [PDF]