HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Schuchardt, L. F. Articles by Verwey, W. F. Search for Related Content PubMed PubMed Citation Articles by Schuchardt, L. F. Articles by Verwey, W. F.

Effect of Formalin on in Vitro and in Vivo Antigenicity of Clostridium Perfringens Type D E-Toxin

From the Merck Institute for Therapeutic Research, Rahway, New Jersey

Abstract

This report may be summarized as follows: a) Toxin-toxoid which failed to combine with antitoxin produced significantly higher antitoxin titers in sheep than did prototoxin-toxoid. b) Toxin-toxoid loses most of its ability to combine with antitoxin in a relatively short period of time (14 days) although some may be retained for as long as 71 days; prototoxin-toxoid, however, retains its combining and precipitating power for a much longer period of time. c) Both toxin and prototoxin combine with antitoxin. d) In the agar diffusion technique a single common zone of precipitation developed between the antitoxin and prototoxin, toxin and prototoxin-toxoid. Toxin-toxoid failed to develop a visible band at a time when prototoxin-toxoid was fully precipitable. e) When either toxin or prototoxin was exposed to 0.5% formalin the toxicity disappeared within 14 days. The combining power and precipitability disappeared relatively soon in the toxin-toxoid but not in prototoxin-toxoid.