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The Reversible and Irreversible Toxic Effects of Antihela Cell Rabbit Serum upon Human Cell Lines in Tissue Culture¹

Department of Microbiology, University of Virginia School of Medicine, Charlottesville, Virginia

Abstract

1. Anti-HeLa cell rabbit serum may produce a variety of cytotoxic effects upon the following cell lines in tissue culture: HeLa, AU, T17, and T27.

2. Irreversible cytotoxic changes are produced when anti-HeLa cell rabbit serum and a heat labile substance in normal guinea pig or rabbit serum are added to these cell lines.

3. Reversible morphologic changes in human cell lines are produced when incubated for 2 hr with anti-HeLa cell rabbit serum.

4. Marked depression of the growth rate of HeLa cells occurs when they are propagated in a medium containing anti-HeLa cell rabbit serum.

5. The addition of complement destroys cultures of HeLa cells propagated for up to six months in an excess of anti-HeLa cell rabbit serum.

Footnotes

This investigation was supported by the Research and Development Fund, University of Virginia School of Medicine, and by a Research Grant C-3173 from the National Cancer Institute, U. S. Public Health Service.

² This investigation was conducted during the tenure of a Damon Runyon Cancer Research Fellowship.