HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Hsiung, G. D. Articles by Melnick, J. L. Search for Related Content PubMed PubMed Citation Articles by Hsiung, G. D. Articles by Melnick, J. L.

Adsorption, Multiplication, and Cytopathogenicity of Enteroviruses (Poliomyelitis, Coxsackie, and Echo Groups) in Susceptible and Resistant Monkey Kidney Cells¹

From the Section of Epidemiology and Preventive Medicine, Yale University School of Medicine, New Haven, Connecticut

Abstract

Experiments with virus adsorption were designed in an attempt to elucidate the differences in susceptibility to the enteroviruses of patas and rhesus monkey kidney cells. This study indicates that the greater number of poliovirus plaques appearing on patas monolayer was due to cell susceptibility rather than to differences of adsorptive capacity among the virus particles.

All the poliomyelitis, Coxsackie, and ECHO viruses tested were adsorbed on both rhesus and patas cells regardless of degree of viral susceptibility of the cells. Thus, while the failure to induce plaques may in some instances be due to the failure to adsorb, as reported for poliovirus on South American capuchin monkey kidney cells, this study showed that other factors in the infection cycle may be involved.

Both Coxsackie A9 and ECHO-1, to which patas cells are apparently resistant, did multiply in patas cells to a limited extent when large inocula were used, although cytopathic changes were questionable or minimal. Coxsackie A9 virus could not be detected after its inoculation into patas cultures, even when the inoculum contained 500 rhesus PFU. The virus disappeared at the end of an hour and could not be detected in either the fluid phase or in cells thereafter. Neither virus produced plaques in patas cells under agar nor cytopathic changes in fluid medium when the inoculum was used in low concentration.

Footnotes

¹ This investigation was aided by a grant from The National Foundation for Infantile Paralysis.

² Present address: Virus Research Section, Division of Biologies Standards, National Institutes of Health, Bethesda, Maryland.