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The Isolation in Tissue Culture, Chick Embryo and Suckling Mice of Filtrable Agents from Healthy Dairy Cattle^1,2,

From the Virus Laboratory, Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor Michigan, and the U. S. Department of Health, Education and Welfare, Public Health Service, Communicable Disease Center

Abstract

The isolation of eight filtrable agents from apparently healthy dairy cattle in cattle kidney tissue culture has been described. Evidence has been presented that they are viral in nature, serologically similar, fairly widespread in the cattle population and probably of little importance in human disease. Studies with the partition cell in the ultracentrifuge indicate that they are relatively small particles with a sedimentation constant of 150 to 200 Sved. Their host range includes monkey kidney tissue culture, chick embryos and 1-day-old suckling mice. The appearance of melanin-like granules in the amniotic fluid of pigmented Barred Rock chick embryos is of interest. The isolation of many new viral agents by the techniques outlined should be productive.

Footnotes

This study was supported by a grant from the National Foundation for Infantile Paralysis and in part by a contract with the Office of the Surgeon General, U. S. Army, Washington, D. C., under the sponsorship of the Commission on Influenza, Armed Forces Epidemiological Board.

² This paper was presented in part before the Conference of Public Health Veterinarians on November 15, 1956.

³ Present address, The Thorndike Memorial Laboratories, Boston City Hospital, Boston, Mass.