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The Journal of Immunology, 1957, 78: 380-386.
Copyright © 1957 by The American Association of Immunologists, Inc.

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Indirect Hemagglutination Studies on Salmonellosis of Chickens

John McNeill Sieburth

Animal Pathology Section, Virginia Agricultural Experiment Station, Blacksburg, Virginia

Abstract

1. An indirect hemagglutination study on salmonellosis of chickens was conducted with chicken erythrocytes modified with practical-type crude antigens prepared by heating Salmonella cell suspension.
2. Hemagglutinin titers appeared earlier, were consistently higher, and persisted longer than agglutinin titers.
3. Serologic and cultural responses of chickens marginally infected with S. typhi-murium did not always correlate.
4. The course of agglutinins and hemagglutinins roughly paralleled each other in S. pullorum and S. typhi-murium infected cockerels.
5. Polyvalent modified erythrocytes produced titers similar to those of monovalent modified erythrocytes to sera from chickensexperimentally infected with organisms from 4 Salmonella serogroups.
6. The spread in polyvalent indirect hemagglutinin titers between uninfected chickens and those from a flock naturally infected with S. typhi-murium appeared adequate for diagnostic purposes.







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