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Serial Determinations of Serum Glycoproteins Following Antigen Injection into Previously Immunized Animals^1,2,3,

From the Department of Infections Diseases, School of Medicine, University of California Medical Center, Los Angeles, California

Abstract

The effects of antigen administration on the serum concentrations of total glycoprotein, seromucoid, -globulin polysaccharide, total protein, and -globulin protein have been determined in guinea pigs previously immunized with heatkilled Brucella suis cells. To evaluate the effects of the repeated bleedings normal animals were bled at weekly intervals. Statistically significant increases occurred in all the serum components following antigen administration and remained elevated through the maximal antibody response. Repeated bleedings were found not to be a factor. The results are discussed with reference to possible causes of the increased levels of serum glycoproteins.

Footnotes

¹ Supported in part by research grants from the National Cancer Institute, National Institutes of Health, Public Health Service, C-2368 (C1), and from the Los Angeles County Tuberculosis and Health Association.

² A preliminary report was presented at the fortieth annual meeting of the Federation of American Societies for Experimental Biology, Atlantic City, New Jersey, April, 1956.

³ Due to the lack of a generally accepted nomenclature for the serum glycoproteins, it is advisable to define the terms employed:

Total serum glycoprotein. The protein-bound hexoses of the serum proteins precipitated by an ethanol concentration of 90% by volume at 25°C.

Seromucoid. The hexose moiety of the perchloric acid-soluble, phosphotungstic acid-insoluble proteins occurring in serum.

-Globulin polysaccharide. The hexose components of the protein-bound carbohydrates of the -globulin fraction. It should be noted that an attempt has been made recently to establish uniformity of terminology (15).