HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Yoshino, K. Search for Related Content PubMed PubMed Citation Articles by Yoshino, K.

Infection of One-Day-Old Fertile Hen's Egg with Herpes Simplex Virus¹

From the Division of Viral and Rickettsial Diseases, National Institute of Health of Japan, Tokyo, Japan

Abstract

A method for the inoculation of herpes simplex virus into the blastoderm of 1-day-old fertile hen's egg is described. After infection, embryonic development ceases, but an increment of virus titer occurs in the egg white of such nondeveloping eggs. Infection can be achieved by as little as one infectious unit as judged by titration on the CAM of 13-day-old embryos. Since the appearance of virus in the egg white was irregular, due to the inhomogeneity of the material, this was replaced by yolk saline. With the eggs thus treated, growth curves were obtained, and it could be demonstrated that newly formed virus was released into the yolk saline at an increasingly higher rate with the passage of time up to 5 days following inoculation, while the whole egg titer, presumably representing intracellular virus, showed a sharp rise already at the end of 24 hr.

Practical applications of the techniques are discussed.

Footnotes

¹ This work was partially supported by the fund for scientific researches granted to Dr. Masami Kitaoka, chief of the Division of Viral and Rickettsial Diseases, National Institute of Health of Japan, from the Ministry of Education of Japan.