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The Preparation of a Soluble Immunizing Antigen from Q-Fever Rickettsiae

From Viral and Rickettsial Section, Research Division, American Cyanamid Company, Pearl River, New York

Abstract

1. The preparation of purified suspensions of Coxiella burnetii by a process involving differential centrifugation using washed celite, and centrifugation through 8% KCl solution is described. The purification can be accomplished without any significant loss of rickettsiae as judged by estimations of infectious titer.

2. The separation of a soluble antigen from suspensions of purified rickettsiae by means of ultrasonic vibration is described.

3. The antigen solutions were shown capable of eliciting antibody production in guinea pigs, and to afford the animals at least partial protection against challenge with viable rickettsiae.