HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Colville, J. M. Articles by Morgan, H. R. Search for Related Content PubMed PubMed Citation Articles by Colville, J. M. Articles by Morgan, H. R.

Temperature and Host Cell Factors in the Growth of Influenza Virus (PR8 Strain) in Avian Tissues in Vitro¹

From the M. Herbert Eisenhart Tissue Laboratory, Department of Bacteriology, University of Rochester School of Medicine and Dentistry, Rochester, New York

Abstract

This study demonstrates that with PR8 strain of influenza A virus in tissue cultures of avian embryonic lung, raising the temperature of incubation from 37 to 41°C does not prevent virus multiplication. In contrast to embryonic lung tissue, lung tissue from 2-day hatched chicks gives no clear-cut evidence of virus under any of the conditions studied. Using embryonic tissues, PR8 virus multiplication, as evidenced by a rise in egg infectivity titer, is greatest with lung and chorioallantoic membrane tissue, less marked with skin-muscle, gut and liver tissue, and not detectable in the case of heart tissue.

The maturation of tissues from the embryonic stage to that of the 2-day hatched chick is associated with the loss of capacity to support PR8 virus multiplication. This difference in tissue susceptibility does not appear to be due to alteration of the environmental temperature of the cells from that of the embryo (37°C) to that of the hatched chick (41°C).

Footnotes

¹ This investigation was supported in part by a grant from the National Cancer Institute (C-2223), National Institutes of Health, Public Health Service.

² Fellow of the National Foundation for Infantile Paralysis.