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Incorporation of Glycine-2-C¹⁴ into Homologous Antibody by Rabbit Tissue Slices¹

From the Department of Chemistry, New York University College of Medicine, New York, New York

Abstract

A controlled system has been designed for the in vitro study of antibody synthesis. To avoid difficulties in interpretation, coprecipitation of radioactive impurities has been controlled by comparison of the radioactivity in a homologous specific precipitate to that in a closely related heterologous specific precipitate made in the same medium.

Animals exhibiting the anamnestic response were superior for the in vitro study of antibody synthesis.

Synthesis of radioactive antibody was demonstrated in isolated rabbit spleen, lymph node, lung and liver slices.

Production of radioactive antibody in each organ studied is in accord with the concentration and distribution of cell types believed to be responsible for antibody synthesis.

Footnotes

¹ Aided by grants from the United States Public Health Service.

² The material in this paper was taken from a dissertation submitted by Bernard Katchen to the Graduate School of Arts and Science of New York University in partial fulfillment of the requirements for the degree of Doctor of Philosophy.