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A Study of an Unusual Hemagglutinin in a Pooled Human Serum¹

From the Mt. Sinai Medical Research Foundation and the Department of Pathology, Chicago Medical School, Chicago, Illinois

Abstract

"Routine" antibody screening test of the D-1 serum showed the presence of only the "incomplete" Rh_o antibody.

Trypsin-treated O rh cells agglutinated very strongly in this serum.

The "T-agglutinin" was not present in this serum.

The agglutination tests before and after absorption of the serum with untreated and enzyme-treated Rh-positive and Rh-negative cells show that the agglutination of the trypsinized red blood cells in this serum is due to an agglutinating factor independent of the Rh_o antibody.

This factor was found to be equally reactive at 0°C, 22°C, and at 37°C for 30 minutes, and absorbable, nondialyzable and thermolabile at 65°C for one hour, unlike the reversible agglutinin factor.

Footnotes

¹ Supported in part by grants from the Leukemia Research Foundation, Inc.

² Postdoctoral Fellow, National Institutes of Health, U. S. Public Health Service. Present address: Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee.

³ Fellow, Chicago Medical School.