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The Effect of Ficin on the Agglutination of Human Red Blood Cells

From the Mt. Sinai Medical Research Foundation and the Department of Pathology, Chicago Medical School, Chicago, Illinois

Abstract

1. A concentration of 1.5 mg of ficin per ml of packed cells, a pH range from 4.7 to 7.8, an incubation temperature of 22° to 37°C and an incubation period of 10 to 30 minutes were found to be the optimum conditions for making A, B and Rh-positive cells agglutinable by the homologous "complete" and "incomplete" antibodies.

2. Ficin was found to be more efficient than trypsin and papain for increasing the sensitivity of O Rh-positive, A and B cells in hemagglutination.

3. The intracellular protease activator, L-cysteine-HCl, accelerated significantly the rate of sensitization of O Rh-positive cells by papain, but not by ficin.

Footnotes

¹ Postdoctoral Fellow, National Institutes of Health, U. S. Public Health Service. Present address: Biology Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee.

² Fellow, Chicago Medical School.