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Immunochemical Studies on Blood Groups

XVI. Quantitative Precipitin Reaction of Blood Group B Substances with Human Anti-B Produced by Isoimmunization of Pregnancy¹

From the Departments of Neurology and Microbiology, College of Physicians and Surgeons, Columbia University and the Neurological Institute, Presbyterian Hospital, New York

Abstract

1. Preparations of B substance from human saliva varied in the amount of anti-B precipitable from an anti-B serum produced by isoimmunization of pregnancy although all of their hexosamine was specifically precipitable by excess antibody.

2. B preparations from 2 horses also precipitated this anti-B and were shown by hexosamine analyses to be of comparable purity to the human saliva substances. Horse substances with little or no B activity by hemagglutination inhibition tests precipitated a portion of the anti-B from this antiserum.

3. B substance from pseudomucinous ovarian cyst and a bovine stomach B substance precipitated less antibody from this antiserum and only about one-half of their hexosamine could be specifically precipitated by anti-B.

Footnotes

¹ This work was carried out under the William J. Matheson Commission and in part under grants from the National Institutes of Health, Public Health Service (RG-34).

² Present address: Vincent Research Laboratory, Massachusetts General Hospital, Boston 14, Mass.