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Kinetic Studies on Immune Hemolysis

VI. Resolution of the C'_Y Reaction Step Into Two Successive Processes Involving C'₂ and C'₃¹

From the Department of Microbiology, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Md.

Abstract

1. It has been shown that C'_x and C'_y correspond to C'_1,4 and C'_2,3, respectively.

2. The complex EAC'_1,4 reacts with C'₂ forming a complex EAC'_1,4,2, which in turn reacts with C'₃, yielding an activated or damaged cell, E*, which then undergoes the terminal transformation reaction to a ghost with release of hemoglobin.

3. Mg⁺⁺ is involved in the action of C'₂.

4. The complex EAC'_1,4,2 has been isolated, and its reaction with C'₂ has been studied.

5. In a system containing optimal Ca⁺⁺ and Mg⁺⁺ the reaction between EAC'_1,4,2 and C'₃ is the rate-controlling process. In a Mg⁺⁺ deficient system the C'₂ step is the pacemaker.

6. The complex EAC'_1,4,2 can serve as a substrate for the determination of C'₃.

Footnotes

¹ The present work was supported by grants from the National Science Foundation, the Cone Foundation, and by a contract (121–104) with the Office of Naval Research.