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The Incorporation of Radioactive Phosphorus into Influenza Virus¹

From the Division of Virology, Department of Public Health and Preventive Medicine, School of Medicine, University of Pennsylvania, and The Children's Hospital of Philadelphia

Abstract

Studies have been presented on the labelling of influenza virus with P³² during propagation in the allantoic cavity of chick embryos. The labelled virus has been purified routinely by 2 cycles of adsorption onto and elution from chicken red cells with intermittent dialysis against large volumes of phosphate buffered saline solution.

The degree of radioactivity of the virus progeny could be improved by an increase in the dose of P³² used, by prolongation of the interval between injection of the isotope and infection, and by a reduction in the age of the embryos employed. The ratio between counts per minute and hemagglutinin units of the yields could be increased further by the use of deembryonated eggs instead of intact chick embryos.

It has not been possible to separate the radioactivity from the virus particles by various biological, physical and chemical means.

Chemical fractionation of labelled virus revealed most of the radioactivity in the lipid and nucleic acid fractions.

Footnotes

¹ The work described in this paper has been supported by a grant-in-aid from the Public Health Service and from the Squibb Institute for Medical Research.

² Medical-Research Division, Sharp and Dohme, Inc., Westpoint, Pa.

³ Squibb Institute for Medical Research, New Brunswick, N. J.