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Kinetic Studies on Immune Hemolysis

III. Description of a Terminal Process Which Follows the Ca⁺⁺ and Mg⁺⁺ Reaction Steps in the Action of Complement on Sensitized Erythrocytes^1,2,

From the Department of Microbiology, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, Maryland

Abstract

It has been shown that the two successive C' reaction steps requiring Ca⁺⁺ and Mg⁺⁺, respectively, are followed by a third process which we have termed the terminal intrinsic transformation reaction, in the course of which intact cells release their hemoglobin and become ghosts.

The terminal reaction proceeds at an appreciable rate even at 0°C. It is not influenced by changes in ionic strength, or reaction volume, nor do citrate or EDTA in moderate concentration have any effect.

Footnotes

¹ A preliminary report of this work has been presented to the American Association of Immunologists at the 1953 meeting of the Federated Societies for Experimental Biology in Chicago, Illinois (Federation Proc., 12: 454, 1953).

² The work reported in this communication was supported in part by grants from the National Science Foundation, The American Cancer Society, Sidney M. Cone Foundation, as well as by a contract with the Office of Naval Research (NONR-248-17).