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Separation of Inhibitors of Hemagglutination and Specific Antibodies for Influenza Viruses by Starch Zone Electrophoresis

From the Hospital of The Rockefeller Institute for Medical Research, New York, N. Y.

Abstract

1. Specific antibodies, inhibitor and inhibitor against influenza viruses in serum were separated from each other by starch zone electrophoresis.

2. Specific antibodies migrated only with globulin, inhibitor was associated with the slowly moving globulin, and inhibitor usually migrated with globulins.

3. Antibodies in human serum and inhibitor in human and rabbit serum each appeared to contain at least two components of different electrophoretic mobility and biological specificity.

4. The inhibitor activity of purified serum mucoprotein or acid glycoprotein was found to be due to a trace contaminant.

5. The inhibitor titer of human serum was elevated during the acute phase of various infectious diseases.