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Distribution of Sensitizing Antibody in Human Serum Proteins Fractionated by Electrophoresis-Convection^*

From the Florence R. Sabin Laboratories, Department of Biophysics (contribution No. 25) University of Colorado Medical Center, Denver, Colorado, and The New York Hospital and Department of Medicine, Cornell University Medical College, New York, New York

Abstract

Three sera, prepared from individuals allergic toward cottonseed or ragweed pollen, have each been partitioned into eight fractions by electrophoresis-convection and into two other fractions by salting out with ammonium sulphate. After being assayed for N content and analyzed for electrophoretic composition, each preparation was repeatedly examined for reagins by both the neutralization and the serum-dilution procedures of passive transference. Activity was found to be concentrated in fractions Va, Vb and VI, which were composed of molecules immobilized by electrophoresis-convection at pH 6 or 5.5. These preparations were rich in -globulin and also contained ₁-globulin. Analysis of the relationship between activity and electrophoretic composition of the 10 fractions supported the hypothesis proposed in our earlier report that -globulins were chiefly responsible for reaginicity. Participation of -globulins could not, however, be directly excluded by our studies.

Footnotes

^* This investigation was supported by the Office of Naval Research, the U. S. Public Health Service grants #1526 (C5) and #A 95 C, and by the American Cancer Society.