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Studies on the Complement Function

Apparent Anomalies of the Complement Function^1,2,

From the Division of Laboratories and Research, New York State Department of Health, Albany

Abstract

1. The fixability of guinea pig complement was found to be quite different from that of human C. The observed antibody titer of an immune serum may be raised or lowered by employing for the fixation test one of the several modified C reagents in admixture with normal C. Alterations of normal C component ratios produce C solutions which are quantitatively identical in hemolytic potency but qualitatively different in fixation to an immune complex.

2. A non-additivity of C hemolytic potency units exists under the conditions of partial fixation to a immune complex, and is a function of the extent of that fixation. The unit of hemolytic activity measured before fixation seems to be different from that measured afterward, indicating the necessity to examine more deeply the exact functions of C components in fixation and hemolysis.

3. The addition of increasing amounts of three C components for the estimation of the "titer" of the remaining component results in a continual increase in titer, never quite reaching the limiting asymptotic value. Hemolytic activity would appear to be a function of the active concentrations of all of the components, not only of that component present in "limiting amounts" as postulated previously.

Footnotes

¹ Supported in part by a cancer control grant from the National Cancer Institute of the National Institutes of Health, U. S. Public Health Service.

² Presented in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Chemistry, Rensselaer Polytechnic Institute, Troy, New York.