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Cutaneous Hypersensitivity in Brucellosis

II. Chromatographic Studies on the Skin-Test Antigen¹

From the Department of Bacteriology, University of California, Berkeley, California

Abstract

1. By the use of chromatography and differential solubility in butanol two brucella fractions were separated from the antigen soluble in Lorol, namely, fraction A which represented the skin test antigen and fraction B which was inactive as a skin test antigen. Acid hydrolysis yielded the same 11 ninhydrin-reactive components from both fractions.

2. Antiserum to Br. suis precipitated fraction B according to chromatographic data and bioassay. The supernate from the precipitin tests contained the allergen.

3. In terms of the amount of nitrogen needed for skin tests on experimentally infected animals, fraction A was nonantigenic when injected into rabbits intravenously whereas fraction B readily stimulated the production of agglutinins.

Footnotes

¹ Aided by funds made available through Contract W-18-064-CM-224 between the University of California and the Biological Department, Chemical Corps, Camp Detrick, Frederick, Maryland.