HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Surgalla, M. J. Articles by Dack, G. M. Search for Related Content PubMed PubMed Citation Articles by Surgalla, M. J. Articles by Dack, G. M.

Use of Antigen-Antibody Reactions in Agar to Follow the Progress of Fractionation of Antigenic Mixtures: Application to Purification of Staphylococcal Enterotoxin¹

From the Food Research Institute, The University of Chicago, Chicago, Illinois

Abstract

Antigen-antibody reactions in agar, modified from the technic of Oudin, have been used to determine the minimum number and to some extent relative concentrations of antigens in enterotoxic solutions at various stages of purification. A simple technic has been devised for direct identification of antigens responsible for bands appearing in different tubes. Resolution of superimposed bands has been accomplished by diluting the antigen mixture. An example of quantitative data which can be used to determine relative concentrations of antigens in fractions of a mixture is presented. Some aspects of practical application in the fractionation of mixtures of antigenic materials are discussed. Staphylococcal enterotoxin has not as yet been identified with a precipitating antigen demonstrable by these methods.

Footnotes

¹ This work was supported by contributions from various companies and associations of the food industries.

This article has been cited by other articles:

				M. S. Bergdoll, B. Lavin, M. J. Surgalla, and G. M. Dack Chromatography in the Purification of Staphylococcal Enterotoxin Science, December 5, 1952; 116(3023): 633 - 634. [PDF]