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The Interaction of Influenza Virus and Intact Human Erythrocytes Observed by Replica Technique in the Electron Microscope¹

From The Section of Preventive Medicine, Yale University School of Medicine, New Haven, Conn.

Abstract

Influenza virus particles on the surface of intact human erythrocytes were observed in the electron microscope by means of replica technique. It was found that a solution containing 0.10 M phosphate buffer with added dextrose or serum albumin to make the solution isotonic was more satisfactory for suspending the cells and virus, than was sodium chloride (0.03 to 0.17 M). Following treatment of cells with virus three changes in the surface of the cells were observed: (1), presence of individual virus particles on the surface; (2), localized eroded or pitted areas which may contain single or clumped virus particles; and (3), a general roughening of the cellular surface. Single cells may show all three types of change.

Footnotes

¹ Aided by a grant from the Fluid Research Fund of the Yale University School of Medicine and representing work done in part under the auspices of the Commission on Virus and Rickettsial Diseases, Armed Forces Epidemiological Board, Office of The Surgeon General, U. S. Army, Washington, D. C.