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The Size of Coxsackie Viruses and Lansing Poliomyelitis Virus Determined by Sedimentation and Ultrafiltration¹

From the Section of Preventive Medicine, Yale University School of Medicine, New Haven, Connecticut and the Department of Virus and Rickettsial Diseases, Army Medical Service Graduate School, Washington, D. C.²

Abstract

The sizes of 6 members of the Coxsackie, or C, group of viruses, representing 4 antigenic types, have been determined by filtration through gradocol membranes and by sedimentation in the ultracentrifuge. Filtration end points and sedimentation constants were measured by following the activity of the infectious particles.

The Conn.-5, Ohio-1, High Point, and Texas-1 C viruses and the Lansing poliomyelitis virus are of comparable size: 15 to 23 mµ according to filtration data and approximately 24 to 32 mµ from sedimentation data. By optical analysis in the sector cell of the ultracentrifuge, the purified Texas-1 strain yielded a value of about 33 mµ. These results are also in agreement with those found by electron microscopy of purified Texas-1 strain.

The TT strain (Type 1) yielded a value of 7 to 10 mµ from the filtration experiments and of about 27 mµ from the sedimentation data.

The complement fixing antigen was found to sediment at about the same rate as the infectious particle for the High Point strain.

Footnotes

¹ Aided by a grant from the National Foundation for Infantile Paralysis.

² Preliminary experiments on the size of these viruses were carried out by the senior author in Stockholm, Sweden, during the summer of 1949 when he held a Fellowship of the American-Scandinavian Foundation at the Caroline Institute, Department of Virus Research, Prof. Sven Gard, chairman. At that time one of the ultracentrifuges at the Institute of Physical Chemistry, University of Uppsala was kindly made available.