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Specificity of Radioiodinated (I¹³¹) Immune Globulin As Determined by Quantitative Precipitin Reaction

From the Laboratory of Experimental Oncology, National Cancer Institute, National Institutes of Health, Public Health Service, Federal Security Agency, and the Division of Medicine, University of California School of Medicine, San Francisco, California

Abstract

1. The reaction between human serum albumin and I¹³¹ trace iodinated rabbit antibody was studied by the quantitative precipitin reaction.

2. The amount of radioactivity found in each immune precipitate was proportional to the antibody nitrogen precipitated.

3. Under the iodinating conditions described (1.3 iodine atoms per antibody molecule) no loss of immunologic specificity of rabbit antibody labelled with I¹³¹ was demonstrated.

Footnotes

¹ Atomie Energy Commission Postdoctoral Fellow in the Medical Sciences of the National Research Council.