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Biophysical Studies of Blood Plasma Proteins

XI. Immunological and Electrophoretic Studies of Immune Chicken Serum¹

From the Departments of Physiological Chemistry and Chemistry, University of Wisconsin, Madison, and the Department of Microbiology, New York University College of Medicine, New York City

Abstract

Introduction. The serum of chickens lacking a -globulin of the normal mammalian electrophoretic mobility offers an unusual system for immunological studies (1–7). In this report there is presented a study of some chemical and biological responses of young chickens to immunization with human serum ₂-globulin (HG). In addition we have investigated the elimination from the circulation of relatively large amounts of homologous serum proteins, obtained by our ethanol fractionation procedures, following their intravenous administration.

Methods and Matherials. Electrophoresis experiments:—These experiments were performed in pH 8.6 veronal buffer of ionic strength 0.1 at a potential gradient of 4.0–4.5 volts/cm. Except as otherwise indicated, the duration of these experiments was three hours. Only descending patterns were used for analyses and for the diagrams to be presented.

Preparation and properties of (HG) and chicken serum protein fractions:—The antigen, HG, was obtained by methods previously described (8).

Footnotes

¹ This work was supported in part by grants from the Wisconsin Alumni Research Foundation, Eli Lilly and Company, and the United States Public Health Service.

² Eli Lilly and Company Research Fellow.

³ William Hallock Park Fellow (New York University) 1948–49.

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