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Precipitin-Tests in Plasmodium Lophurae Malaria¹

Department of Public Health and Preventive Medicine, Cornell University Medical College, New York

Abstract

1. Specific precipitin- and collodion-particle-agglutination tests are described with antigens and sera from ducks infected with Plasmodium lophurae.

2. A method for production of a relatively stable antigen from the plasma of ducks heavily infected with P. lophurae is outlined.

3. Antigen is present in two fractions of the plasma from ducks heavily infected with P. lophurae, but the fraction obtained from the second acid-precipitation (pH 3.2) is more potent and stable than that obtained from the first (pH 5.6 ±).

4. In general, precipitin-formation follows the appearance of parasites in the peripheral blood and thereafter may follow, coincide with, or vary inversely as the parasitemia.

5. In ducks that recover, it appears that precipitins persist in the blood for 1 to 2 weeks and then are no longer measurable.

6. Recovery from infection was always associated with precipitin-formation, but precipitin-formation was not always followed by recovery.

Footnotes

¹ This investigation was aided by the Marcelle Fleischmann Foundation.

² The senior author spent the last two years of his life in the study of this problem, leaving the clear, concise notes from which it was possible to construct this report. That this was possible compels acknowledgement of the high order of governed, scientific intelligence that had labored so carefully and well. It was his final research and was finished just before his death.