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Studies on Streptomycin

V. Complex Formation between Streptomycin and Cellulose, Proteins, and Desoxyribonucleic Acid and Its Reversal by Salt

Sam Berkman^*, Riley D. Housewright and Richard J. Henry^*,,

From the Biological Division, Chemical Corps Research and Development Department, Camp Detrick, Frederick, Maryland

Abstract

Streptomycin is adsorbed by cellophane (pure cellulose) and can be eluted by solutions of sodium chloride.

Studies with dialysis indicate that streptomycin forms a complex with albumin and with one or more components of horse serum. The albumin complex retains the full activity of free streptomycin, while there is some loss of streptomycin activity in the horse serum complex.

Complex formation between desoxyribonucleic acid or calf thymus nucleoprotein and streptomycin is accompanied by a shift toward the higher wave lengths in the ultra-violet absorption peak characteristic of nucleic acid. It is postulated that the formation of complexes involves a change in resonance. Reversal of these spectral changes was effected by sodium chloride.

Footnotes

^* Present address: Bio-Science Laboratories, Inc., 10717 Venice Blvd., Los Angeles, Calif.

With the technical assistance of Louise A. Brough.