HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Cooper, M. L. Articles by Keller, H. M. Search for Related Content PubMed Articles by Cooper, M. L. Articles by Keller, H. M.

Studies in Dysentery Vaccination^*

IV. Primary Vaccination of Children with Monovalent Vaccines of Shigella

From the Children's Hospital Research Foundation, and the Department of Pediatrics, College of Medicine, University of Cincinnati

Abstract

Six different monovalent vaccines were administered subcutaneously to groups of children 13 to 19 years of age.

One monovalent vaccine was administered subcutaneously, intravenously, and intradermally to three groups of children.

Intravenous administration of vaccine stimulated not only the best homologous antibody response but also the broadest statistically significant heterologous antibody response.

Subcutaneous administration of vaccine stimulated the next best antibody response.

Homologous antibody response was always greater than heterologous antibody response.

Statistically significant homologous antibody response persisted as long as followed in the sera of children given adequate amounts of vaccine. This was six weeks in the case of one vaccine, ten weeks for another, twenty-four weeks for each of four others, and fifty-two weeks for another.

Crossing antibody response was greatest between Shigella flexner I-V, II-W, III-Z, and VII-X.

Heterologous antibody response also occurred between Shigella flexner I-V and Shigella fiexneri VI-Boyd 88.

There was no statistically significant heterologous antibody response between Shigella sonnei and the strains of Shigella flexneri.

There was no heterologous antibody response between Shigella ambigua and the strains of Shigella flexneri.

The antigenic and toxic reactions to these vaccines were related directly to the content of bacterial cells or bacterial nitrogen.

There is evidence of parallelism between toxicity and antibody response.

Shigella vaccine standardized by viable bacterial count to contain ten billion killed bacteria per ml usually stimulated systemic reactions which were too severe.

Shigella vaccine standardized to contain 7.5 mg N/100 ml was not sufficiently antigenic.

We found the passive mouse protective test more sensitive than the agglutinative test for differentiating greater degrees of immune response.

A polyvalent vaccine composed of Shigella fiexneri II-W, III-Z, VI-Boyd 88, Shigella sonnei and Shigella ambigua should stimulate significant antibody response against Shigella flexneri I-V, I I - W , I I I - Z , VI-Boyd 88, VII-X, Shigella sonnei and Shigella ambigua.

Footnotes

^* This study was conducted under a contract with the Office of Scientific Research and Development.