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Ultraviolet Absorption Spectroscopy of Immune Precipitates

From the Department of Medicine, New York University College of Medicine, New York, N. Y.

Abstract

1. The ultraviolet absorption spectrum of a rabbit antibody has been recorded.

2. The spectrum does not change appreciably over a broad range of the precipitin curve.

3. UV absorption provides an accurate, extremely sensitive method for the quantitative measurement of immune precipitates.

4. Analysis of spectral data offers, within limits, an opportunity to determine the antigen-antibody composition of those immune precipitates whose components have significant and different UV absorption spectra.

Footnotes

¹ Senior Research Fellow, National Institute of Health.