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Some Factors Influencing the Selection of a Complement-Fixation Method

II. Parallel Use of the Direct and Indirect Technics

Division of Animal Pathology, Science Service, Dominion Department of Agriculture ( Animal Diseases Research Institute, Hull, Quebec, Canada)

Abstract

A method (indirect complement-fixation test) is described for titrating the inhibitory activity of non-complement-fixing antisera when mixed with homologous antigen and complement-fixing antisera from another animal species.

The possible application of the method in surveys of the antibody content in diversified avian and mammalian populations is suggested as well as in situatins in which marked discrepancies are recorded between the results of the usual complement-fixation tests and other serologic procedures.