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Studies on Acute Respiratory Infections¹

X. The Nature and Value of a So-Called Precipitin Reaction As Applied to the Serological Grouping of Streptococci

From the Bureau of Laboratories, Department of Health, New York City

Abstract

Barnes, (1), in a study on the cultural and serological relationship of hemolytic streptococci reported recently that the serums of rabbits intensively immunized against the streptococcus will give precipitin reactions with streptococcus antigens even when such serums are diluted as high as 1-3200.

His method of eliciting what he terms a precipitin reaction, was as follows: The streptococci were grown in broth for forty-eight hours at 37°C. The broth cultures were centrifuged at high speed to sediment the cocci. In each test a constant amount (1 cc.) of clear undiluted supernatant broth antigen was added to an equal amount of the diluted antistreptococcus serum. These mixtures as well as controls with normal rabbit serum and with uninoculated broth were incubated at 37°C. for eight to ten hours, removed to the ice-box for two hours and then read. Sterility tests were made to eliminate the possibility of the precipitate being bacterial growth.

Footnotes

¹ One of a series of studies carried out under the direction of William H. Park, Anna W. Williams and Charles Krumwiede. The previous studies in this series were reported in this Journal, 1921, vol. 6, no. 1. This investigation was made possible by a grant of money from the Metropolitan Life Insurance Influenza Fund for a part of the expense.