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The Journal of Immunology, 1921, 6: 329-342.
Copyright © 1921 by The American Association of Immunologists, Inc.

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Studies on Acute Respiratory Infections1

IX. Differences in the Character of the Hemolytic Action of Streptococci and the Relative Value of Various Methods in Demonstrating These Differences

Lucy Mishulow

From the Bureau of Laboratories, Department of Health, City of New York

Abstract

Differences in the hemolytic activity of streptococci when grown on blood agar were noted by Schottmuller (1) in 1903. The blood-agar plate was generally employed to determine the hemolytic ability of streptococci until Lyall (2) (1914) advised the use of the tube method as a means of differentiation. Later Smith and Brown (3), using the blood agar plate method, introduced a new nomenclature to indicate the reactions obtained. Brown (4) elaborated these findings and gave detailed descriptions of the characteristics to be observed and advocated a classification based upon such observations. He gave a very complete review of the whole subject.

The superiority of either the plate method or the tube method depends upon the answers to the following questions: (a) Does the plate method bring out all the differential characteristics not shown by the latter method, or is the reverse true? (b) Does each method bring out certain characteristics?

Footnotes

1 This study is part of an investigation on acute respiratory diseases carried on under a grant from the Influenza Commission Fund of the Metropolitan Life Insurance Company. This series of studies was carried out under the direction of William H. Park, Anna W. Williams and Charles Krumwiede.







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